Untargeted Metabolomics
Untargeted metabolomics is an unbiased approach to detect all endogenous small-molecule metabolites in a sample. It utilizes bioinformatics analysis methods to study the overall changes and metabolic characteristics of the endogenous metabolome in response to perturbations, aiming to identify the endogenous small-molecule metabolites responsible for such metabolic differences and explore the underlying metabolic mechanisms. Compared to targeted metabolite profiling, untargeted metabolomics provides more comprehensive and objective information, avoiding the loss of important data and directional biases. It is commonly used for discovering metabolic profile differences and differential metabolites.
Platform and Strategy
Platform and Strategy GC-MS/LC-MS
Technical workflow
Bioinformatics analysis pipeline and partial results presentation
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生物信息分析流程图
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| 差异代谢物相关性热图 | 差异代谢物层次聚类分析 | 差异代谢物富集代谢通路气泡图 |
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| 正交偏最小二乘法判别分析 | 差异代谢物火山图 | 差异代谢物KEGG通路分析 |
Application areas
1、Medical Field: Untargeted metabolomics is primarily used to investigate the dynamic changes in small-molecule metabolites within the body, analyze the relationship between metabolites and physiological or pathological changes in order to study disease mechanisms, identify biomarkers, and more. It has applications in clinical diagnosis, pathophysiological research, disease phenotyping, drug development and screening, drug effic. monitoring, drug effic. and toxicity evaluation, preclinical animal model selection, etc.
2、Food Health and Nutrition Field: Untargeted metabolomics is applied towards achieving personalized dietary recommendations, promoting the development of molecular toxicology in food safety, and has broad applications in food traceability and ingredient evaluation. It is used in food processing development, dietary recommendations, food origin tracing, food authenticity testing, nutritional composition optimization, etc.
3、Agriculture and Forestry Field: By studying all small-molecule metabolites in plants of different species, genetic types, or ecological types during different growth stages or before and after certain stimuli, or investigating the effects of different environmental stressors on plants, metabolic patterns can be discovered. It has applications in the development of pesticides and herbicides, plant stress response research, plant genetics and breeding, genetically modified organisms, etc.
4、Livestock Industry: Untargeted metabolomics is utilized to elucidate the characteristic changes in animal metabolism caused by factors such as nutrition supply, feeding conditions and methods, different feeds, and veterinary drugs. It helps uncover the mechanisms of action within animals and contributes to improving the efficiency and ensuring the safe and stable development of the livestock industry. Its main applications include scientific feeding, disease prevention and treatment, developmental research, etc.